Insertional inactivation using Lac Z gene (Blue white screening)
11:33
Khan AcademyKhadijatul Qubra
Insertional inactivation using Lac Z gene (Blue white screening)
In this video, let's explore how we can use an enzyme-producing selectable marker to select for the right cells. We will take the example of the Lac Z gene, which naturally digests Beta-galactosidase, and see how using X-gal, we can screen for the recombinants. This method takes fewer steps than using multiple antibiotic markers, and is quicker ...
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